Human F13B(Coagulation Factor XIII B Polypeptide) ELISA Kit
Human F13B(Coagulation Factor XIII B Polypeptide) ELISA Kit
Description
| Reference | ENT-E6850 |
|---|---|
| Category | Human F-N |
| Size | 96T*5 |
| Assay Type | Competitive Inhibition |
| Sensitivity | 1.24 ng/mL |
| Standard | 200 ng/mL |
| Detection Range | 3.13-200 ng/mL |
| Sample Type | plasma |
| Assay Lenght | 2h |
Research Area: Signal transduction;Metabolic pathway;Hematology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human F13B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F13B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F13B in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: F13-B; FXIIIB; Fibrin-stabilizing factor B subunit; Protein-glutamine gamma-glutamyltransferase B chain; Transglutaminase B chain