Human ET-1(Endothelin1) ELISA Kit
Human ET-1(Endothelin1) ELISA Kit
Description
| Reference | ENT-E13418 |
|---|---|
| Category | Human A-E |
| Size | 48T |
| Assay Type | Sandwich |
| Sensitivity | 2.73 pg/mL |
| Standard | 500 pg/mL |
| Detection Range | 7.82-500 pg/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Lenght | 3.5h |
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Research Area: Endocrinology;Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1