EasyStep Human VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit
EasyStep Human VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit
Description
| Reference | ENT-E2185 |
|---|---|
| Category | Human A-E |
| Size | 96T*5 |
| Assay Type | Sandwich |
| Sensitivity | 31 pg/mL |
| Standard | 5000 pg/mL |
| Detection Range | 78.2-5000 pg/mL |
| Sample Type | Serum, plasma |
| Assay Lenght | 1.5h |
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Research Area: Cytokine;Apoptosis;Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGFA, and the Human VEGFA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VEGFA. After TMB substrate solution is added, only those wells that contain Human VEGFA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGFA in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor