Human Anti-GAD(Anti-Glutamic Acid Decarboxylase Antibody) ELISA Kit
Human Anti-GAD(Anti-Glutamic Acid Decarboxylase Antibody) ELISA Kit
Description
| Reference | ENT-E31657 |
|---|---|
| Category | Human A-E |
| Size | 96T*5 |
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.59 ng/mL |
| Standard | 100 ng/mL |
| Detection Range | 1.57-100 ng/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Assay Lenght | 2.5h |
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Research Area: Enzyme & Kinase;Endocrinology;Neuro science;Autoimmunity;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-GAD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-GAD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-GAD in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: Glutamate Decarboxylase