Human 5-HT(5-Hydroxytryptamine) ELISA Kit
Human 5-HT(5-Hydroxytryptamine) ELISA Kit
Description
| Reference | ENT-E33238 |
|---|---|
| Category | Human A-E |
| Size | 96T |
| Assay Type | Competitive Inhibition |
| Sensitivity | 4.05 ng/mL |
| Standard | 900 ng/mL |
| Detection Range | 14.07-900 ng/mL |
| Sample Type | serum, plasma |
| Assay Lenght | 2h |
PDF File: https://enteksci.com/wp-content/uploads/2025/10/ENT-E33238.pdf
Research Area: Signal transduction;Infection immunity;Neuro science;Gastroenterology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 5-HT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: 5HT; Serotonin; N-Methyl-Gamma