Human 3-MH(3-Methylhistidine) ELISA Kit
Human 3-MH(3-Methylhistidine) ELISA Kit
Description
| Reference | ENT-E9199 |
|---|---|
| Category | Human A-E |
| Size | 96T*5 |
| Assay Type | Competitive Inhibition |
| Sensitivity | 2.61 nmol/mL |
| Standard | 400 nmol/mL |
| Detection Range | 6.25-400 nmol/mL |
| Sample Type | serum, plasma, tissue homogenates and other biological fluids |
| Assay Lenght | 2h |
PDF File:
Research Area: Metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 3-MH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 3-MH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 3-MH in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: 3MH