Human DA(Dopamine) ELISA Kit
Human DA(Dopamine) ELISA Kit
Description
| Reference | ENT-E37229 |
|---|---|
| Category | ELISA HUMAN |
| Size | 48T |
| Assay Type | Competitive Inhibition |
| Sensitivity | 4.53 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Lenght | 2h |
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Research Area: Signal transduction;Cardiovascular biology;Reproductive science;Neuro science;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human DA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DA in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: 2-(3,4-Dihydroxyphenyl)ethylamine; 3,4-Dihydroxyphenethylamine; 3-Hydroxytyramine; Intropin; Revivan; Oxytyramine; Dopamine Hydrochloride