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Human p?(phospho Tau Protein) ELISA Kit

Human p?(phospho Tau Protein) ELISA Kit

Human p?(phospho Tau Protein) ELISA Kit

Description

Reference ENT-E37217
Category ELISA HUMAN
Size 48T
Assay Type Sandwich
Sensitivity 5.8 pg/mL
Standard 1000 pg/mL
Detection Range 15.63-1000 pg/mL
Sample Type Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght 3.5h

PDF File: 

Research Area: Signal Transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human p?. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human p?. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human p?, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human p? in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: p?; AI413597; AW045860; DDPAC; FLJ31424; FTDP 17; G protein beta1/gamma2 subunit interacting factor 1; MAPT; MAPTL