Human FSTL3(Follistatin Like Protein 3) ELISA Kit
Human FSTL3(Follistatin Like Protein 3) ELISA Kit
Description
| Reference | ENT-E37193 |
|---|---|
| Category | ELISA HUMAN |
| Size | 48T |
| Assay Type | Sandwich |
| Sensitivity | 0.112 ng/mL |
| Standard | 20 ng/mL |
| Detection Range | 0.32-20 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Lenght | 3.5h |
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Research Area: Reproductive science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FSTL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FSTL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FSTL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FSTL3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: FLRG; FSRP; Follistatin-Related Protein,Secreted Glycoprotein; Follistatin-related gene protein