Recombinant Human FOLR1 (C-6His)
Recombinant Human Folate RecESCItor Alpha is produced by our Mammalian expression system and the target gene encoding Arg25-Ser234 is expressed with a 6His tag at the C-terminus.
Description
| Reference | ESCIT288 |
|---|---|
| Size | 50ug |
| Molecular Weight | 25.7 KDa |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Biological Activity | Immobilized Human FOLR1-His(Cat#C784) at 1?g/ml (100 ?l/well)can bind Anti-Human FOLR1 mAb(Cat#NC072).The ED50 of Anti-Human FOLR1 mAb(Cat#NC072)is 0.626 ng/ml. |
Other names: Folate recESCItor alpha;FR-alpha;Adult folate-binding protein;FBP;Folate recESCItor 1;Folate recESCItor;Ovarian tumor-associated antigen MOv18;FOLR1
Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100?g/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Background: Folate recESCItor alpha(FOLR) belongs to the folate recESCItor family, and is primarily expressed in tissues of ESCIithelial origin. It is also expressed in kidney, lung and cerebellum. The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. It has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after recESCItor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. It is required for normal embryonic development and normal cell proliferation.