Biotinylated Human CD3E (C-Fc-Avi)
Biotinylated Recombinant Human T-cell Surface Glycoprotein CD3 ESCIsilon Chain is produced by our Mammalian expression system and the target gene encoding Asp23-Asp126 is expressed with a Fc, Avi tag at the C-terminus.
Description
| Reference | ESCIT267 |
|---|---|
| Size | 20ug |
| Molecular Weight | 40.5 KDa |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Biological Activity | Loaded Anti-Human CD3E mAb-mFc(Cat#NC079) on AMC Biosensor, can bind Human CD3E-Fc-6His&C-Fc-Flag(Cat#CY33) with an affinity constant of 0.6 nM as determined in BLI assay. |
Other names: T-Cell Surface Glycoprotein CD3 ESCIsilon Chain; T-Cell Surface Antigen T3/Leu-4 ESCIsilon Chain; CD3e; CD3E; T3E
Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100?g/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Background: T-Cell Surface Glycoprotein CD3 ? Chain (CD3?) is a single-pass type I membrane protein. CD3? contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3? is a polypESCItide encoded by the CD3E gene on chromosome 11 in humans. The T cell recESCItor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and rESCIresents one of the most complex transmembrane recESCItors. The T cell recESCItor-CD3 complex is unique in having ten cytoplasmic immunorecESCItor tyrosine-based activation motifs (ITAMs). TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.