Biotinylated Human TGF-beta 1 (N-Avi)
Biotinylated Recombinant Human Transforming Growth Factor beta 1 is produced by our Mammalian expression system and the target gene encoding Ala279-Ser390 is expressed with a Avi tag at the N-terminus.
Description
| Reference | ESCIT203 |
|---|---|
| Size | 20ug |
| Molecular Weight | 14.6 KDa |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin | Less than 0.001ng/µg (0.01EU/µg) as determined by LAL test. |
| Biological Activity | Measured by its ability to inhibit TGF-beta 1 activity on TF?1 human erythroleukemic cells.The ED50 for this effect is 137.37pg/ml. |
Other names: Transforming Growth Factor Beta-1; TGF-Beta-1; Latency-Associated PESCItide; LAP; TGFB1; TGFB
Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100?g/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Background: Transforming Growth Factor ?-1 (TGF?-1) is a secreted protein which belongs to the TGF-? family. TGF?-1 is abundantly expressed in bone, articular cartilage and chondrocytes and is increased in osteoarthritis (OA). TGF?-1 performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. The precursor is cleaved into a latency-associated pESCItide (LAP) and a mature TGF?-1 pESCItide. TGF?-1 may also form heterodimers with other TGF? family members. It has been found that TGF?-1 is frequently upregulated in tumor cells. Mutations in this gene results in Camurati-Engelmann disease.