Recombinant Mouse HGF (C-6His)
Recombinant Mouse HESCIatocyte Growth Factor is produced by our Mammalian expression system and the target gene encoding Gln33Leu728 is expressed with a 6His tag at the C-terminus.
Description
| Reference | ESCIT121 |
|---|---|
| Size | 10ug |
| Molecular Weight | 53.5&26.9 KDa |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin | Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
| Biological Activity |
Other names: HESCIatopoietin-A; Scatter factor; SF; deafness; autosomal recessive 39; DFNB39; F-TCF; hESCIatocyte growth factor
Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100?g/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Background: HGF, is a pleiotropic protein in the Plasminogen subfamily of S1 pESCItidases. Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propESCItide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. HGF binds hESCIaran-sulfate proteoglycans and the widely expressed recESCItor tyrosine kinase, HGF R/c-MET. HGF regulates ESCIithelial morphogenesis by inducing cell scattering and branching tubulogenesis. It can also alter ESCIithelium morphology by the induction of nectin?1 alpha ectodomain shedding, an adhesion protein component of adherens junctions .