Recombinant Human HGF (C-6His)
Recombinant Human HESCIatocyte Growth Factor is produced by our Mammalian expression system and the target gene encoding Gln32-Ser728 is expressed with a 6His tag at the C-terminus.
Description
| Reference | ESCIT082 |
|---|---|
| Size | 50ug |
| Molecular Weight | 26&53.7 KDa |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin | Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
| Biological Activity | Measured by its ability to induce IL-11 secretion by Saos?2 human osteosarcoma cells. The ED50 for this effect is 0.3-1.5 ng/ml. |
Other names: HESCIatocyte growth factor; HPTA; HGF; SF; Scatter factor; HESCIatopoietin-A
Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100?g/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Background: HGF, is a pleiotropic protein in the Plasminogen subfamily of S1 pESCItidases and contains 4 kringle domains, 1 PAN domain and 1 pESCItidase S1 domain. HGF is secreted as an inactive 728 amino acid (aa) single chain propESCItide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. HGF binds hESCIaran-sulfate proteoglycans and the widely expressed recESCItor tyrosine kinase, HGF R/c-MET. HGF regulates ESCIithelial morphogenesis by inducing cell scattering and branching tubulogenesis. It can also alter ESCIithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions. HGF regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto oncogenic c-Met recESCItor. HGF is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly ESCIithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration.