Recombinant K. pneumoniae NEO
Recombinant Klebsiella Pneumoniae Aminoglycoside 3′-phosphotransferase is produced by our E.coli expression system and the target gene encoding Met1-Phe264 is expressed.
Description
| Reference | ESCIT008 |
|---|---|
| Size | 50ug |
| Molecular Weight | 29 KDa |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Biological Activity |
Other names: Aminoglycoside 3′-phosphotransferase; APH(3′)-II; APH(3′)II; Kanamycin kinase type II; Neomycin-kanamycin phosphotransferase type II; neo
Redissolve:
Storage: Store at ?-70°C, stable for 6 months after receipt.
Store at ?-70°C, stable for 3 months under sterile conditions after opening.
Please minimize freeze-thaw cycles.
Background: Aminoglycoside 3′-phosphotransferase (APH(3′)), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dESCIendent enzymes phosphorylate the 3′-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH(3′) is primarily found in certain species of gram-positive bacteria.