Dbf4 rabbit pAb
ENT-A2571
Description
| REF | ENT-A2571 |
|---|---|
| Category | Antibody Polyclonal |
| Description | Dbf4 rabbit pAb |
| Source | Rabbit |
| Applications | WB;IHC;IF;ELISA |
| Reactivity | Human;Mouse;Monkey |
| Reactivity | Human;Mouse;Monkey |
| Dilution | Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DBF4. AA range:10-59 |
| Storage Stability | -20°C/1 year |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Observed Band KD | 77kD |
| Human Gene ID | 10926 |
| Human Swiss Prot Nº | Q9UBU7 |
| Subcellular Location | Nucleus . |
Other Name: DBF4; ASK; DBF4A; ZDBF1; Protein DBF4 homolog A; Activator of S phase kinase; Chiffon homolog A; DBF4-type zinc finger-containing protein 1
Background: function:Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at ‘Ser-40’ and ‘Ser-53’ and then is involved in regulating the initiation of DNA replication during cell cycle.,induction:Induced in G1 phase at low level, increased during G1-S phase and remain high during S and G2-M phase.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 DBF4-type zinc finger.,similarity:Contains 2 BRCT domains.,subunit:Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2L, ORC4L and ORC6L.,tissue specificity:Highly expressed in testis and thymus. Expressed also in most cancer cells lines.,