Calregulin rabbit pAb
ENT-A2307
Description
| REF | ENT-A2307 |
|---|---|
| Category | Antibody Polyclonal |
| Description | Calregulin rabbit pAb |
| Source | Rabbit |
| Applications | WB;Flow Cyt;IHC;IF;ELISA |
| Reactivity | Human;Mouse;Rat;Monkey |
| Reactivity | Human;Mouse;Rat;Monkey |
| Dilution | WB 1:500-2000;Flow Cyt 1:50-200;IHC-p 1:100-500;IF/ICC 1:100-500;ELISA 1:5000-20000 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CALR. AA range:21-70 |
| Storage Stability | -20°C/1 year |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Observed Band KD | 48kD |
| Human Gene ID | 811 |
| Human Swiss Prot Nº | P27797 |
| Subcellular Location | Endoplasmic reticulum lumen . Cytoplasm, cytosol . Secreted, extracellular space, extracellular matrix . Cell surface . Sarcoplasmic reticulum lumen . Cytoplasmic vesicle, secretory vesicle, Cortical granule . Cytolytic granule . Also found in cell surface (T cells), cytosol and extracellular matrix (PubMed:10358038). During oocyte maturation and after parthenogenetic activation accumulates in cortical granules. In pronuclear and early cleaved embryos localizes weakly to cytoplasm around nucleus and more strongly in the region near the cortex (By similarity). In cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation (By similarity). . |
Other Name: CALR; CRTC; Calreticulin; CRP55; Calregulin; Endoplasmic reticulum resident protein 60; ERp60; HACBP; grp60
Background: Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its