NFκB-p65 (phospho Ser536) rabbit pAb
ENT-A1879
Description
| REF | ENT-A1879 |
|---|---|
| Category | Antibody Polyclonal |
| Description | NFκB-p65 (phospho Ser536) rabbit pAb |
| Source | Rabbit |
| Applications | IF;WB;IHC;IP;ELISA |
| Reactivity | Human;Mouse;Rat;Monkey |
| Reactivity | Human;Mouse;Rat;Monkey |
| Dilution | IF: 1:50-200 Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser536. AA range:502-551 |
| Storage Stability | -20°C/1 year |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Observed Band KD | 60kD |
| Human Gene ID | 5970 |
| Human Swiss Prot Nº | Q04206 |
| Subcellular Location | Nucleus . Cytoplasm . Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B) (PubMed:1493333). Colocalized with DDX1 in the nucleus upon TNF-alpha induction (PubMed:19058135). Colocalizes with GFI1 in the nucleus after LPS stimulation (PubMed:20547752). Translocation to the nucleus is impaired in L.monocytogenes infection (PubMed:20855622). . |
Other Name: RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Background: NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],