PARP Mouse mAb
ENT-A1492
Description
| Ref | ENT-A1492 |
|---|---|
| Category | Antibody Monoclonal |
| Alternative Name | PARP-1, Poly(ADP ribose) polymerase 1, sPARP1,ADPRT1, ADP ribosyltransferase NAD(+) |
| Application | WB, IHC |
| Source | Mouse |
| Reactivity | Human |
| Dilution | WB 1:1,000-3,000 IHC 1:200-500 |
| Immunogen | Synthetic Peptide |
| Storage Stability | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Concentration | 1mg/mL |
| Observed Band(KD) | 116kDa |
| Human Gene ID | 142 |
| Human Swiss Prot No | P09874 |
| Subcellular Location | Nucleus |
Background: Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks. Knocking down intracellular PARP1 levels with siRNA or inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks. In the absence of PARP1, when these breaks are encountered during DNA replication, the replication fork stalls, and double-strand DNA (dsDNA) breaks accumulate.