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β-Tubulin Mouse mAb

β-Tubulin Mouse mAb

ENT-A1409

Description

Ref ENT-A1409
Category Antibody Monoclonal
Alternative Name
Application WB, IHC, IF
Source Mouse
Reactivity Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit,Sheep, Insect, Yeast
Dilution WB 1:5,000 IHC 1:200 IF 1:100-200
Immunogen Synthetic Peptide
Storage Stability PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality Monoclonal
Isotype IgG1
Concentration 1mg/mL
Observed Band(KD) 55kDa
Human Gene ID 203068
Human Swiss Prot No P07437
Subcellular Location Cytoplasm,Cytoskeleton,Microtubule

Background: Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.