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GAPDH Mouse mAb

GAPDH Mouse mAb

ENT-A1408

Description

Ref ENT-A1408
Category Antibody Monoclonal
Alternative Name
Application WB, IHC, IF
Source Mouse
Reactivity Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep, Insect, Yeast
Dilution WB 1:5,000 IHC 1:200
Immunogen Synthetic Peptide
Storage Stability PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality Monoclonal
Isotype IgG1
Concentration 1mg/mL
Observed Band(KD) 37kDa
Human Gene ID 2597
Human Swiss Prot No P04406
Subcellular Location Cytoplasm,Cytoskeleton,Membrane,Nucleus

Background: Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.