Human CA19-5(Carbohydrate Antigen 19-5) ELISA Kit
Human CA19-5(Carbohydrate Antigen 19-5) ELISA Kit
Description
| Reference | ENT-E28954 |
|---|---|
| Category | Human A-E |
| Size | 96T*5 |
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.85 ng/mL |
| Standard | 200 ng/mL |
| Detection Range | 3.13-200 ng/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Assay Lenght | 2h |
Research Area: Tumor immunity;Hepatology;Gastroenterology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CA19-5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA19-5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA19-5 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: CA19-5