Human ASMA(Anti-Smooth Muscle Antibody) ELISA Kit
Human ASMA(Anti-Smooth Muscle Antibody) ELISA Kit
Description
| Reference | ENT-E36051 |
|---|---|
| Category | Human A-E |
| Size | 96T |
| Assay Type | Sandwich |
| Sensitivity | 3.2 ng/mL |
| Standard | 500 ng/mL |
| Detection Range | 7.82-500 ng/mL |
| Sample Type | tissue homogenates, cell lysates and other biological fluids |
| Assay Lenght | 3.5h |
PDF File: https://enteksci.com/wp-content/uploads/2025/10/ENT-E36051.pdf
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ASMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ASMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ASMA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ASMA in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: ASM Ab; Anti Smooth Muscle Antibody