Human Anti-MBL(Anti-Mannose Binding Lectin Antibody) ELISA Kit
Human Anti-MBL(Anti-Mannose Binding Lectin Antibody) ELISA Kit
Description
| Reference | ENT-E32086 |
|---|---|
| Category | Human A-E |
| Size | 96T*5 |
| Assay Type | Sandwich |
| Sensitivity | 2.54 ng/mL |
| Standard | 400 ng/mL |
| Detection Range | 6.25-400 ng/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Assay Lenght | 3.5h |
Research Area: Signal transduction;Infection immunity;Immune molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-MBL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-MBL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-MBL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-MBL in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)