Human Anti-AsAb(Anti-Sperm Antibody) ELISA Kit
Human Anti-AsAb(Anti-Sperm Antibody) ELISA Kit
Description
| Reference | ENT-E32118 |
|---|---|
| Category | Human A-E |
| Size | 96T |
| Assay Type | Sandwich |
| Sensitivity | 1.19 ng/mL |
| Standard | 200 ng/mL |
| Detection Range | 3.13-200 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Lenght | 3.5h |
PDF File: https://enteksci.com/wp-content/uploads/2025/10/ENT-E32118.pdf
Research Area: Reproductive science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-AsAb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-AsAb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-AsAb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-AsAb in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: Anti-Spermatozoa Antibodies; Sperm Antibodies; Antisperm Antibodies