Human VD3(Vitamin D3) ELISA Kit
Human VD3(Vitamin D3) ELISA Kit
Description
| Reference | ENT-E3687 |
|---|---|
| Category | Human O-Z |
| Size | 96T |
| Assay Type | Competitive Inhibition |
| Sensitivity | 2.07 ng/mL |
| Standard | 400 ng/mL |
| Detection Range | 6.25-400 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Lenght | 2h |
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Research Area: Metabolic pathway;Reproductive science;Nutrition metabolism;Bone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human VD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VD3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: Cholecalciferol; Toxiferol; Activated 7-Dehydrocholesterol