Human TrkA/NTRK1(High affinity nerve growth factor receptor) Microsample ELISA Kit
Human TrkA/NTRK1(High affinity nerve growth factor receptor) Microsample ELISA Kit
Description
| Reference | ENT-E36161 |
|---|---|
| Category | Human O-Z |
| Size | 48T |
| Assay Type | Sandwich |
| Sensitivity | 32 pg/mL |
| Standard | 5000 pg/mL |
| Detection Range | 78.13-5000 pg/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Lenght | 3.5h |
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Research Area: Cell Biology; Neuroscience; Signal Transduction; Developmental Biology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TrkA/NTRK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TrkA/NTRK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TrkA/NTRK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TrkA/NTRK1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: NTRK1; MTC; TRK; TRKA; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor; Tyrosine kinase receptor A;