Human TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit
Human TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit
Description
| Reference | ENT-E34856 |
|---|---|
| Category | Human O-Z |
| Size | 96T*5 |
| Assay Type | Sandwich |
| Sensitivity | 1.14 ng/mL |
| Standard | 200 ng/mL |
| Detection Range | 3.13-200 ng/mL |
| Sample Type | serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| Assay Lenght | 3.5h |
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Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TPO-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TPO-Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: TPO Ab; TPO-Ab