Human TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit
Human TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit
Description
| Reference | ENT-E34956 |
|---|---|
| Category | Human O-Z |
| Size | 48T |
| Assay Type | Competitive Inhibition |
| Sensitivity | 3.58 nmol/mL |
| Standard | 400 nmol/mL |
| Detection Range | 6.25-400 nmol/mL |
| Sample Type | Serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| Assay Lenght | 2h |
Research Area: Metabolic pathway;Hepatology;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TBARS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TBARS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TBARS in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: TBARS; Thiobarbituric Acid Reactive Substance