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Human SST(Somatostatin) ELISA Kit

Human SST(Somatostatin) ELISA Kit

Human SST(Somatostatin) ELISA Kit

Description

Reference ENT-E21558
Category Human O-Z
Size 48T
Assay Type Competitive Inhibition
Sensitivity 2.55 pg/mL
Standard 500 pg/mL
Detection Range 7.82-500 pg/mL
Sample Type Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Lenght 2h

PDF File: 

Research Area: Tumor immunity;Endocrinology;Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human SST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SST in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: GHIH; SRIF; SMST; GHRIH; GHRIF; Growth Hormone Releasing Inhibiting Hormone; Somatotropin Release-Inhibiting Factor; Somatostatin-14; Somatostatin-28